Categories
Type
DEL
Application note
The document describes the integration of focal Molography with DNA-encoded library (DEL) technology to streamline the screening and validation of small molecule hits in drug discovery. This method allows simultaneous kinetic characterization of up to 54 compounds in a single step, enhancing efficiency and enabling detailed insights into binding dynamics in physiologically relevant environments.
Crude interactions
Application note
The experiments highlighted the method’s reliability in differentiating molecular interactions in both PBS-T buffer and human serum. Using anti-GFP VHH, the technique demonstrated comparable kinetic parameters and sensitivity. The robustness against non-specific effects and its compatibility with physiological conditions make focal molography valuable for biological and therapeutic research.
DEL
Application note
Teh document shows the integration of focal molography with DNA-encoded library (DEL) technology, streamlining drug discovery by enabling efficient screening and kinetic characterization of small molecules. This approach accelerates the identification of potential drug candidates, offering a high-throughput, robust platform for studying molecular interactions and advancing therapeutic development.
Focal molography
Publication
Blickenstorfer, Y., Borghi, L., Reichmuth, A. M., Fattinger, C., Vörös, J., & Frutiger, A. (2021). Sensors and Actuators B: Chemical, 349, 130746.
Focal molography
Publication
Blickenstorfer, Y., Müller, M., Dreyfus, R., Reichmuth, A. M., Fattinger, C., & Frutiger, A. (2021). Physical Review Applied, 15(3), 034023.
Crude interactions
Publication
Focal molography
Publication
Frutiger, A., Fattinger, C., & Vörös, J. (2021). Sensors, 21(2), 469.
Focal molography
Publication
Frutiger, A., Gatterdam, K., Blickenstorfer, Y., Reichmuth, A. M., Fattinger, C., & Vörös, J. (2020). Sensors, 21(1), 9.
Cell interaction
Publication
Reichmuth, A. M., Zimmermann, M., Wilhelm, F., Frutiger, A., Blickenstorfer, Y., Fattinger, C., ... & Vörös, J. (2020). Analytical chemistry, 92(13), 8983-8991.